Media And Growth Of Bacteria

Media And Growth Of Bacteria To grow and reproduce, microorganisms require nutrients as their source of energy and certain environmental conditions. Adaptation to different habitats has been acquired by microbes living in the exposed environment. In the laboratory, however, these requirements are to be met by a culture medium. Therefore, media preparation is an essential aspect in microbial growth. There is a wide variety of media which can be used for laboratory purposes. Generally, similar procedures can be used in the preparation of media. In this practical, YT broth is prepared from yeast extract, trypton, NaCl and agar. YT broth is a commonly used bacterial Esherichia coli culture media in molecular biology. Usually, YT medium is applicable for phage DNA production. Compared to LB broth, YT is a richer medium in which it supports higher cell density and a longer growth period for E. coli. Bacteria are the most abundant and most important biological component as they involve in the transformation and mineralization of organic matter in the biosphere (Cho Adam, 1988; Pomeroy Wiebe, 2001). Generally, growth is defined as an increase in number of cells. Bacterial cell growth depends upon a large number of cellular reactions such as transforming energy. Syntheses of small molecules are performed to build macromolecules and to provide various cofactors and coenzymes for biochemical reactions. Polymerization of monomers builds the macromolecules in the bacterial cell. As the macromolecules accumulate in the cell, they are assembled into new structures with specific functions such as the cell wall, cytoplasmic membranes, ribosomes and flagella, if to name a few, and eventually lead to cell division. E. coli is used as a test organism in this practical for investigating the bacterial growth. Such a growing rod shaped cell bacterium elongates to approximately twice their original length and then forms a partition that constricts the cell into two daughter cells. This process is known as binary fission. A partition called the septum is formed by the inward growth of the cytoplasmic membrane and the cell wall from opposite directions. The septum continues to grow inwards until the two daughter cells are pinched off. When one cell separates from to form two daughter cells, one generation has occurred and the time required for binary fission is known as the generation time. Under the best nutritional conditions, the generation time of a laboratory E. coli culture is approximately 20 minutes (Buettner et al., 1973). The growth stages of bacterial cells are described in a growth curve consisting of the lag phase, exponential phase, stationary phase and death phase (Zwietering et al., 1990). In the lag phase, the bacterial cell begins to grow only after a period of time due to new environment being inoculated. The time interval of lag phase depends on the growth conditions and the history of the inoculum. If the growing culture is transferred into the same medium under the same conditions, there is no lag phase and exponential phase starts immediately. However, if the inoculum transferred is taken from the stationary phase in an old culture, lag phase is observed. This is because certain essentials of the cell have depleted and time is required for biosynthesis of new constituents. The lag phase is also observed when the cells are transferred from a rich culture medium to a poorer culture medium. In the poorer condition, the cells need to synthesize the essential metabolites that are not present i n the previous medium. Thus, time is needed by the cells to produce new enzymes. In the exponential phase, the cell divides to form two cells, both the total cell number and mass double but there is no change in the average cell mass. The cells are said to be in their healthiest state. The rate of exponential growth varies widely among microorganisms. The rate is influenced by environmental conditions and the genetic characteristics of the organism itself. In the stationary phase, the essential nutrients of the culture medium are used up and at the same time, the wastes produced by the bacterial cells accumulate in the culture medium. Thus, growth is inhibited. The exponential growth is ceased and the cells reach the stationary phase. There is no net increase or decrease in the cell number and hence the growth rate of the cells is zero. The cells enter the death phase of the growth cycle after reaching the stationary phase whereby cell lysis occurs. Figure 1: Typical growth curve of a bacterial population. The growth of E.coli is investigated under various cultural conditions, including effect of pH, temperature and aeration). Effect of pH is ascertained by preparing media of different pH values. Temperature is always a potentially limiting factor by affecting all chemical and biochemical processes and thus is viewed as an interactive factor (Pomeroy Wiebe, 2001). Aeration is done by shaking so that air space volume can be increased. As such, good and excellent aeration is stimulated. MATERIALS Media components (yeast extract, trypton, NaCl and agar), E. coli culture, 20x petri dish, 5250 ml conical flask and 1100 ml conical flask, plus cotton wool plugs in aluminium foil, Whatman paper METHOD Media Preparation The five 250 ml conical flasks and one 100 ml conical flasks were prepared and labelled as M1-M10 on the first day of practical. 100 ml of YT broth was prepared in 250 ml flasks, with the composition as trypton 1.6%(w:v), yeast extract 1%(w:v), and NaCl 0.5%(w:v). The pH was adjusted to those shown in table 2.1, followed by autoclaving. 500 ml agar was prepared in 1L flask, in which composed of YT broth and agar (13.5 g/L). The prepared medium was autoclaved. The agar was poured on 20 petri dishes. Inoculation Day 2 was begun with inoculating M1 to M8 with 1.0ml E. coli, while M9 and M10 as control set. Incubation was performed at 37Â °C for 24 hours. Whatman paper was dried overnight. Analysis Prior to an analysis, the culture was well shaken and the sampling was done under aseptic condition. Measurement of cell density and variable cell (as colony forming units, cfu) After the overnight incubation, a 3.0ml aliquot of each culture was taken and the absorbance was measured at 660nm. M9 and M10 was used as the blank. Data obtained was tabulated. Measurement of final pH of broth The final pH of the cell culture was measured. The results were also recorded in table 2.1. Measurement of CFU At day 3, a serial dilution was done from 10-2, 10-4, 10-6, 10-8 to 10-10. Hockey stick spreading technique was performed to plate out the sample. Two replicates were done for each plate, labelled a and b respectively. The culture was incubated at 37Â °C for 24 hours. The changes were observed and recorded on the next day, and thereby determining the CFU, as what recorded in table 2.2(a) and 2.2(b). Measurement of biomass The Whatman paper dried was weighed and the initial weight was recorded. A 10ml aliquot of the content of the flasks was filtered using the Whatman paper by washing with distilled water. The paper together with the content was dried in oven at 70Â °C overnight. On the next day, the final weight was measured and thereby obtaining the net weight gain of the filter paper. The result was recorded in table 2.3. RESULTS The pH change and the absorbance values measured were recorded in table 2.1. Flask M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 Pre-incubation pH 4.0 7.0 10.0 7.0 4.0 7.0 10.0 7.0 7.0 7.0 Post-incubation pH 3.96 7.08 10.16 7.16 4.13 7.21 9.93 7.73 7.12 7.14 OD660nm 0.108 1.267 0.01 0.916 0.191 1.906 0.056 1.812 0.0 0.0 Growth condition No shaking (with air space) No shaking (without air space) Shaking at 200 rpm (with air space) Shaking at 200 rpm (without air space) Table 2.1: pH change and absorbance measured for M1 to M10 CFU for plate labelled a and for plate b was calculated using the formula below, which was then recorded in table 2.2(a) and 2.2(b) respectively. CFU = colony number x dilution factor = x cfu/0.1ml = ? cfu/ml Table 2.2(a): CFU for plate labelled a Flask M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 Dilution (10-4) 55 22 0 >300 1 161 0 >300 0 0 CFU/ml 5500000 2200000 0 TMTC 100000 16100000 0 TMTC 0 0 Dilution (10-6) 0 13 0 185 0 24 0 3 0 0 CFU/ml 0 130000000 0 1850000000 0 240000000 0 30000000 0 0 Flask M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 Dilution (10-4) 13 >300 0 >300 0 >300 0 >300 0 0 CFU/ml 1300000 TMTC 0 TMTC 0 TMTC 0 TMTC 0 0 Dilution (10-6) 0 3 0 89 0 32 0 4 0 0 CFU/ml 0 30000000 0 890000000 0 320000000 0 40000000 0 0 Table 2.2(b): CFU for plate labelled b The biomass was calculated using the formula below and was recorded in table 2.3. Biomass (g/ml) = Flask M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 Initial weight of filter paper 1.14 1.16 1.14 1.17 1.19 1.15 1.16 1.19 1.16 1.17 Dried weight of filter paper 1.15 1.17 1.14 1.17 1.20 1.17 1.16 1.19 1.16 1.17 Mass Difference 0.01 0.01 0.0 0.0 0.01 0.02 0.0 0.0 0.0 0.0 Biomass (mg /ml) 0.001 0.001 0.0 0.0 0.001 0.002 0.0 0.0 0.0 0.0 Table 2.3: Biomass DISCUSSION From the tables in the result section and observation, we analyze the growth of E. coli under various pH and aeration condition. Measuring OD and calculating CFU give relevant and supportive information to our experiment. However, the biomass calculated is not applicable to accurately ascertain the factors affecting the growth as some errors occurred during the measurement of biomass, probably. Cell density and variable cell OD stands for optical density which indicates the wavelength of light. In this practical, OD660 measures the light absorbance by E. coli cultures, which correspond to the E. coli cell density in a given volume. OD660 determines whether the cells are ready for making cell stocks or as a competent cell. In other words, it can be said that in this part, OD660 was used to ascertain the quantity of E. coli grown in the YT broth after incubating overnight at 37Â °C. Flasks M1 to M8 were inoculated with 1.0 ml of E. coli culture. The result showed that growth had occurred. On the other hand, M9 and M10 served as the control set in which inoculation of bacteria was not applied, in addition to acting as blank during analysis with spectrophotometer. From table 2.1, it was shown that the OD of both M9 and M10 appeared to be zero. This shows that there was no sign of growth of E. coli in the two flasks. Spreading of plates was done in class II laminar flow cabinet. Sterile operation in cell culture is vital where it can affect the CFU of E. coli. This minimizes the chance of culture contamination. On top of that, the safety of the operator is ensured (Betler, 2004). From the aspect of CFU, as what shown in table 2.2(a) and 2.2(b), there was abundant of cells in M2, M4, M6 and M8. The cell density was still high even with the dilution of 10-6. Therefore, it was determined that E. coli has the optimum growth pH at 7.0. Effect of broths pH on growth Different growth condition gives different OD values. Optimum pH of E.coli growing in a culture at 37oC is around 7.0. E.coli is not able to tolerate under extremely alkaline and acidic environments because most of the enzymes found in the bacterium are very pH-sensitive. These enzymes carry out the important processes in E.coli. When there is extreme change in pH, enzymes in E.coli become denatured. Denature of enzymes in E.coli can lead to all sorts of interruptions to biochemical processes or even causes death of the E.coli. From table 2.1, it was found that there was no growth of E.coli in both flask M3 and M7 with pH 10.0; as the absorbance measured were 0.01 and 0.056 respectively. Whereas, there were some E.coli grow in flask M1 and M5 with pH 4.0, which have OD recorded 0.108 and 0.191 respectively. Thus, it can be concluded that E.coli seems to be more tolerate to lower pH (acidic condition) than higher pH (alkaline condition). Since pH 7.0 is the optimum pH for the growth of E.coli at 37oC, there were many colony of E.coli growing on the agar plates which spread with the sample from flask M2, M4, M6 and M8. There are some changes in the pH before and after the incubation. This is because there is formation of inhibitory products such as organic acids during the growth of E.coli in the YT nutrient broth. Effect of aeration on growth pH of medium is determined by medium composition, buffers, cellular metabolism and aeration. NaCl is used as buffer, where the depletion of this organic buffer will cause an E. coli reaches its maximum pH limit. Hence, care has to be taken to balance the organic buffer, sugar content and aeration medium because low aeration leads to production of acids; High aeration causes cells to use organic acids as carbon source, and thus increases the pH of medium. In brief, selected aeration also helps in maintaining cells pH. Theoretically, shake flask incubator reduces the solid and liquid inter-phase, thus minimizing the mass transfer. Increased speed and momentum cause cells to lyse among themselves. Increased speed also increases the formation of foam, thus trapping gas and resulting less gas in the liquid. Since cells require nutrients for survival, absence of gas as nutrient source will cause cells to die. Hence, shaking flask actually plays an important role in bringing an improvement to mass transfer between the gas phase outside the shake flask and the liquid phase inside the shake flask. Shake flask incubator can provide a suitable environment by ensuring sufficient transfer of oxygen gas to the cell culture by appropriate mixing. Agitation will increase the aeration of the bacterial growth of E.coli. This is because oxygen is important for high density growth of E.coli cells. Agitation is controlled by the shaking speed of a shaker incubator. Thus, from the observation, Whatman paper with content from M6 has bigger biomass than others. The biomass of M6 is 1.0 mg/ml. This is because flask M6 has been shaken at 200rpm and the flask contains air space which helps in the growth of E.coli cells. Therefore, the media without shaking or without air space or without both of these growth conditions has less growth of E.coli. Thus, M6 has the best growth condition for E.coli if compared with other media. However, from the observation in this experiment, there is more E.coli colony grows in M8 (growth condition with shaking at 200rpm but without air space) than M6 (growth condition with both shaking at 200rpm and with air space) in the culture with serial dilution 10-4. Theoretically, there should be less E.coli colony grows on the plate which spread with the culture from M8 since flask M8 has less flavour growth condition for the growth of E.coli. This unexpected observation may be because the sample with serial dilution 10-4 is not well shaken before take 1.0ml of aliquot to spread on the plate. From our result, it was again determined that the growth rate of E. coli is not affected by shaking. This is proven through our experiment where there was no significant difference in yields with or without shaking cultures. When air is supplied, however, shaking cultures shows a higher yield than static cultures. The main purpose of shaking cultures at 20 rpm is to mix thoroughly the nutrient concentration in broth, with constant temperature, 37Â °C. 37Â °C is the optimum growth temperature for E.coli. Shaking prevents cells localizing at the bottom of the broth which causes a faster depletion of nutrient as time passes. Nutrients still present in the broth but cells may not accessible to them. Hence, shaking minimizes areas of high and low nutrient concentration. The amplitude of the vibration controls the intensity of fluid mixing. Shaking broth brings advantage to non-motile cells because it enables the metabolism rate to continue, in addition to benefiting mobile cells by redu cing energy on mobility. Besides, shaking increases the aeration of broth which leads to a higher yield of E. coli. From the OD values, OD at pH 7 shows the highest value for both shaking and non-shaking media because pH 7 is the most suitable medium for growth of E.coli. However, pH 7 from shaking broth has a comparatively higher OD reading compare to non-shaking broth due to shaking enhances the growth of E.coli. However, OD of pH 4 is far higher than OD of pH 10 because E.coli can still grow on pH 4, but pH 10 does not really support growth of E.coli. As for M9 and M10, both broths do not show any OD reading value because no E.coli culture is added into both broths. When pH and vibration are held constant (i.e. pH 7 with shaking), broth with aeration shows a higher OD value compare to non-aeration. This means that a higher E.coli cell density in aerated broth compare to non-aerated broth. When other factors are held constant, air space does not significantly affect E.coli yields, because E.coli is a facultative anaerobe, which means E.coli is able to produce energy during aerobic respiration and switch to anaerobic respiration when oxygen is depleting in the environment. Thus, E.coli grows in both with or without air space broth. Supposedly, colony forming unit (CFU) in plates from medium with air space has a higher growth amount compare to medium with no air-space. This is because E.coli cells produce a large quantity of acetic acid with no air-space, and thus reducing the pH of broth and does not favour the growth of E.coli. Acetic acid inhibits growth condition under anaerobic growth condition. However, according to our result, when the pH and vibration are held constant (both of pH 7 and with no shaking), CFU is greater in non air space compare to with air space. This may be due to errors occur where the mouth of broth is not tightly covered which leads to entranc e of oxygen molecules into the broth. CONCLUSION Sterile medium such as sterile YT broth is essential for bacteria growth. The optimum pH for the growth of E. coli is 7.0. Excellent aeration promotes growth of bacteria to a further extent than what good aeration does. QUESTIONS Write a flow chart for the above protocol before you start work. Label all the conical flasks from M1 to M10 Add 100ml YT broth to flasks M1 to M3 and M5 to M7 Adjust the pH of the media to 4.0,7.0 and 10.0 using either HCL or NaOH Autoclave and allow to cool to room temperature For flask M4 to M8, autoclave 200ml of the medium in a bigger container and then pour the cooled medium into the sterile 100ml conical flask Inoculate flasks M1 to M8 with 1.0ml of E.coli culture under aseptic conditions Incubate with or without shaking as indicated from the table Prepare M9 and M10 under the same conditions as for flask M2 and M6, but do not add any inoculum Prepare and autoclave the agar After an overnight incubation,take 3.0ml aliquot of each culture and measure the absorbance at 660nm Do a serial dilution from 10-1 to 10-5 in a sterile microfuge tube and plate out 0.1ml of the sample at 10-4 to 10-5 in YT agar media Incubate at 370C overnight Determine the cfu/ml of the original culture and tabulate data Measure the final pH of the cell culture and tabulate results Filter 10ml aliquot of the content of the flasks using pre-weighted Whatman paper dried in an oven at 800C overnight Measure the net weight gain of the filter paper as an indication of biomass Tabulate results Discuss your results with respect to the effect of pH of the media and aeration on bacterial growth and reasons for your observations. In addition to nutrients, the pH of the growth medium is also important for E.coli growth rate and cell density. The optimal growth pH for E.coli is near neutral. E.coli cells can grow reasonably well over a range of three pH units (from pH 5.5 to 8.5). Extreme pH beyond this range will significantly decrease the cell growth rate and may sometimes even cause cell death. The minimum and maximum growth pHs for E.coli are pH 4.4 and 9.0 respectively. E.coli cells appear to tolerate a more neutral pH better than a high pH. This is clearly shown as E.coli grows well at pH 7 as shown on plate M2, M4,M6, and M8.In fact, extended exposure of E.coli cells to a high pH causes cell lysis. This is the reason why no colonies were found on plate M3 and M7. At the saturation or stationary phase, the pH of the E.coli culture in commonly used media is near its pH limits. pH is another limiting factor for cell growth in addition to nutrition exhaustion and accumulation of toxic metabolites. E.coli cel ls can also use sugars such as glycerol and glucose as carbon or energy sources. When the E.coli cells use these sugars as carbon sources, they will produce acetic acid and therefore lower the medium pH. Carefully balancing the sugar contents, and aeration conditions can maintain the culture medium pH near E.coli optimum growth pH or within the range of the three pH units. Low aeration conditions lead the cells to produce acids. High aeration conditions allow the cells to use organic acids as carbon source and increase medium pH. Selected aeration conditions can also help cells maintain its medium pH. Do your data on absorbance at 660nm correlate with the cfu/ml and biomass data? Explain. The data on the absorbance at 660nm correlates with cfu/ml as a lower OD660nm gives a high cfu/ml and vice versa. But the data of absorbance does not correlate with the biomass. Despite the inherent inaccuracy of the method, if the procedure is adequately controlled and calibrated the estimation of microbial numbers by optical density should be sufficiently accurate for use in preparing inoculum for cfu/ml testing. Would you expect to obtain the same data if you were to scale up the experiment using a 1000L fermenter? Explain your answer. No, as the size and volume of the fermentor increases, the volume and density of the E.coli culture will increase too so the CFU/ml will also increase.

My Philosophy of Education Essay -- Philosophy on Teaching Statement

Philosophy of Education One characteristic of a good teacher is a sound philosophy of education. This will ensure the readiness of the teacher in the classroom setting. The philosophy should be thought out with care and contain concrete ideas concerning several aspects of education. My philosophy of education contains my view about: the nature of students, the nature of knowledge, the purpose of public education, teaching methods, and curriculum. People have many different views about the nature of students. Like Rousseau, I feel that students are neither good nor evil. The situations they encounter in their everyday lives shape the way they behave and the person they become. The nature of students isn’t always shaped by money or social status. For example, a poor student may grow up to set a good example for society. They may secure a good position at a successful job regardless of social class. On the other hand, a socially affluent student may grow up and become a criminal. Experiences determine which path every student will take. Students can choose to make the best of their circumstances or let these circumstances control their fate. Children from single parent homes can choose to let their situation be an excuse for lack of progress, or they can choose not to let it stand in their way. I feel that circumstances such as being from a single parent home or losing a parent aren’t sufficient reasons n ot to succeed. Some students may choose to use them as such, but in reality, these are not excuses at all. Rousseau felt that children should only be exposed to the good experiences. In my opinion, this may make the child weak and leave him or her to deal with later life events. I also agree with Rousseau... ...cular career choices. However, all students should have a general understanding of basic scientific processes. Some everyday items can be created through basic scientific knowledge. Fire extinguishers can be created rather quickly if needed for a small emergency. Giving a plant the appropriate proportions of water, sunlight, and fertilizer can be assessed through a basic understanding of some scientific principles. Teachers have many responsibilities to themselves and their students. They are responsible for the knowledge their students gain and sometimes the paths they take. A teacher can â€Å"make or break† a student. Students will respect a good teacher and admire them. They are willing to learn from a good teacher. A sound philosophy and a knowledge of what the teacher expects from his or her self is a step in the right direction with regard to a career.

Marriot and Body Shop Aims and Objectives Essay

introduction Marriot is an international brand however the purpose of this controlled assessment, they will be my local business known as London Heathrow Marriott-LHM. Marriott is currently a private limited company (PLC) and has two other private investors as owners of the company. The Marriott hotel was founded in 1993 and is located in Harlington, Hayes. Currently its labour turnover for the month of September (2010) is an increase of 10.1%. Marriott acquires approximately 133,000 employee’s world wide and between 8,000-9,000 employees in the UK. My other local business is The Body shop. The Body Shop is an international business Definition of Aims The long-term goals a business wants to achieve. Goals that a business wants to achieve to be successful. Explain what aims are Definition of objectives The specific steps taken by a business to achieve a goal Objectives have to S.M.A.R.T, this stands for: * Specific * Measurable * Achievable * Realistic * Time Marriott’s aims * Guest Satisfaction Superiority * Marriott Profitability & Owner Return on Investment * Associate Satisfaction Marriott’s objectives * GSS (Customer Target) 75% (satisfied with overall service) * Sales turnover of  £20m per annum * Labour turnover less than or equal to 25% Summarising Marriott’s aims and objectives Marriot have set themselves three aims that they hope to achieve. These three aims vary from making a profit on their investment to guest and employee satisfaction. Marriott’s first aim and objective focuses on customer satisfaction. They are going to meet this goal by being customer focused and meeting customer needs. To achieve this they will do everything in their power to keep their customers happy to give recommendations to other people and for them to keep coming back to their hotel. They need to deliver the â€Å"wow factor† by providing the best quality of service. They intend to meet this aim by encouraging their guests to complete a â€Å"Guest Satisfaction Survey†. To achieve their aim they need 75% of their customers to be satisfied. The management team reviews all these surveys to find out what they need to improve on to make the hotel and make it the best in the industry. They also focus on associate satisfaction which focuses on the employees. They are striving for labour turnover less than or equal to 25% therefore Marriott hope not to lose more than 1 in 4 of their employees. They aim to make employees satisfied by ensuring their staff is happy. They will achieve this aim by motivating their staff and rewarding them with staff benefits for example LHM offer â€Å"Valuable room rate, food and beverage, and retail discounts at global Marriott locations.† This gives the employees the feeling that Marriott cares about them. Also the amount of pay that is offered by Marriott is higher than most equivalent jobs. This makes the employees want to stay as they know that there is a very slim chance that they will find this amount of pay anywhere else, or they may not get the same amount of bonuses-motivating them to do better- The want the communication between the staff and Marriott management to be strong as this gives the employees the sense that they are involved within the business. Their third aim and objective focuses on Income and money made. Marriott want to make a profit and have a sales turnover of  £20m per annum. By doing this they will be able to maintain share prices and reward shareholders with dividends. Furthermore they will be able to do this by increasing sales revenue for this hotel. This has been set out by the management & senior Marriott personnel in the US. The will achieve this by providing the best quality of service. body shopAims * Leading business in the beauty industry * Fair trade * Protect human rights – customers and staff * Protect the Environment and planet body shop objectives * Continue to grow by maximizing sales * All our suppliers have signed the Code of conduct supporting ethical, fair trade program. * Improving working conditions for body shop employees and protecting their Human rights * Every product packaging is made from 100% recycled materials Summary of the body shop aims and objectives

Comparison of Early American Literature Essay Example

Comparison of Early American Literature Essay Example Comparison of Early American Literature Essay Comparison of Early American Literature Essay The two choices Of Plymouth Plantation by William Bradford and The General History of Virginia by John Smith are some of the earlier pieces of American literature. Although they were both written in the same clip period the manner and attitudes vary greatly. William Bradford had a really direct signifier of authorship ; normally known as plain puritan manner. whereas. John Smith had a slightly confusing. more luxuriant. composing manner. Bradford’s piece besides seemed more accurate than John Smith’s history. For illustration. John Smith wrote of his gaining control by †¦three 100 archers. conducted by the male monarch of Pamunkee†¦ which seems more than a small overdone. After all. it by and large doesn’t take three hundred work forces to capture one. Smith exaggerated many times in order to tout about himself. There are many times when he refers to his illustriousness. In the undermentioned quotation mark he boasts of his leading accomplishments and compassion for his fellow work forces while minimizing his higher-ups: The new President and Martin. being small beloved. of weak judgement in dangers. and less industry in peace. committed the managing of all things abroad to Captain Smith. who. by his ain illustration. good words. and just promises. put some to cut down. others to adhere thatch. some to construct houses. others to thatch them. himself ever bearing the greatest undertaking for his ain portion. so that in short clip her provided most of them diggingss. pretermiting any for himself†¦ William Bradford. on the other manus. self-praises about his settlement: †¦there was but six or seven sound individuals who to their great citations. be it spoken. spared no strivings dark or twenty-four hours. but with copiousness of labor and hard of their ain wellness. fetched them wood . made them fires. dressed them meat. made their beds. washed their nauseating apparels. clothed and unclothed them. Bradford and Smith. both leaders of their settlements. wrote of their adversities in the new universe. Despite these similarities the manner they acted was really different. When John Smith writes about the Native Americans he refers to them as savages and barbarians. He even calls Pocahontas. the miss who saved his life. a immature dame. Bradford writes of the Native Americans as human existences. He even had a peace pact with them that lasted twenty-four old ages. They besides had different motives for authorship. which may lend to the many differences. Smith wrote his choice to promote people to come to America to happen exhilaration and escapade. Bradford merely wanted to inform the readers of what the lives of settlers was truly similar. As different are their authorship manners. motives. and positions these two work forces portion some common land. one being they are some of the earliest plant of American Literature. So no affair how different or similar Smith and Bradford’s Hagiographas are. History of Virginia and Of Plymouth Plantation will ever be remembered as great American literature.

Elderly Depression Example

Elderly Depression Example Elderly Depression – Term Paper Example Elderly Depression Depression is one of the major psychological problems found among elderly people. â€Å"It has been estimated that perhaps somewhere between 10 and 30 percent depressives and manic depressives kill themselves† (Klein, 3rd edition (September 8, 2009) 3. Klein, Donald F.M.D Revised edition (May 12, 2005) 4. Miller, Mark D M.D 1 edition (September 23, 2002)

Music therapy Essay Example | Topics and Well Written Essays - 2750 words

Music therapy - Essay Example Musical therapy is a health profession. It is an interpersonal process where the therapist, who is trained personnel, develops a relationship with his clients using music and all of its facets i.e. physical, emotional, mental, spiritual, and aesthetic aspects, enabling them to improve their health. They use musical experiences such as singing, songwriting, and listening, free improvisation, discussing to music and even moving to music to improve the healing process. The health issues in subject consist of cognitive functioning, emotional and affective development, behavioral and social skills, motor skills and, the quality of life. Music therapists can work in general hospitals, psychiatric facilities, schools, prisons, community centers, universities, and training institutes. Music therapist is in the hospital setup; work hand in hand with the physicians, psychologists, physical therapists and the occupational therapists. Individuals of all ages and those with specific requirements such as the stroke, sensory impairments, communication disorders, cancer, psychiatric disorders, those in palliative care and those in rehabilitation centers (recovering from substance abuse) benefit a lot form music. Apart from the entertainment they get from the music, they also get relaxed, improve their learning, build their self-esteem, reduce stress, support physical exercise and get composed which improves the healing process. Music therapy has its history back in the biblical times when David played the harp to get rid of an evil spirit, which was in King Saul. In 400, B.C, the Greek father of medicine played music to his mental patients. Music therapy began after World War I and II, where musicians would travel to hospitals to play music to soldiers who suffered from war-related trauma. Aristotle himself described music as an energy that purifies the emotions. While music is an art with a strong connection with mathematics, music therapy is an art and a science. Introductio n Music therapy has a strong relationship with play, creativity, use of the whole personality and individual discovery of one self. In fact, Winnicott (2005, p.120) claims that It is in playing and only in playing, which the individual adult or child develops the ability to be creative and utilize the whole personality, and the individual discovers the self only in being creative. Â  This is all that makes music therapy different from other professions. The item one creates should have some value accompanied with it. There exist a relation between personal intelligence and creativity, and every session involves the client in some kind of music experience. The various sessions include improvising, re-creating, composing, and listening to music. In improvising, the client comes up with his/her own music and starts singing whatever arises now.

CCTV argument supporting the idea Essay Example | Topics and Well Written Essays - 1500 words

CCTV argument supporting the idea - Essay Example Undoubtedly, the expansion of globalisation, rise in societal complexities and new forms of criminal activities have accumulatively given rise to this need. Increasing urbanisation has exacerbated the trend towards privacy, leading to the essentiality of verifying identity. The growing frequency of crime has further emerged as one of the major reasons for both international corporations and government to increase video surveillance through CCTV (Ratcliffe & Taniguchi, 2006). Emphasising these aspects, the aim of this essay is to highlight problems arising due to the increasing number of CCTV cameras in the modern society and develop rational understanding in this regard. Need of CCTV Cameras With the advent of globalisation in the modern world, keeping track of regular activities performed within a society has become very essential for the government of any country to ensure national security. CCTVs are used not only in security purposes but also in research where people are continuo usly observed to understand human behaviour in different situations and circumstances. Increased mobility both locally and internationally has further given rise to a stranger society, where social control based on cognitive understanding matters significantly. Risk management has also become the leading mode of reasoning for both international organisations and government organisations, which has further increased the use of CCTV cameras. In the area of criminal justice, new ideas have given way to more humble defensive responses that focus on opportunity reduction, situational avoidance and risk management wherein CCTV can be regarded as a vital part of the trend towards the effective identification of punishing offenders, managing prisoners and handling rehabilitation centres. There are records that show CCTV is more effective in some context than other technology gadgets in identifying crimes. Reports published on the effectiveness of using CCTV have revealed that it had a signi ficant impact on decreasing vehicle crimes, robbery and theft. However, no such significant impact of using CCTV was seen in case of violence or felony (Damjanovski, 2005). Researches further depict that identification of the real offender is possible through the CCTV cameras, which in turn plays a vital role as evidence in any criminal prosecution. Though proper evidence has not yet been obtained in the violence sector, the cameras have been observed to record suspicious behaviours and therefore play a pivotal role in obstructing the conduct of any probable crime to ensure security of a given population. However, the effectiveness of CCTV is often affirmed as subjected to constant monitoring, which could only be done in a reliable manner by a computer programme that searches for unusual activities and highlights it for further investigation. Notably, to increase efficiencies in identifying crime and protecting the society with the assistance of CCTV, innovative software programmes, known as Artificial Intelligence Systems (AIS) are being developed, which can predict the occurrence of crime quite effectively (Ulkemen, 2009). For instance, in a recent crime activity of the â€Å"Boston Marathon Bombing†, the CCTV installed in a departmental store helped the FBI team in identifying the concerned perpetrator of the crime. CCTV has also shown significant contribution in controlling hacking